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NUMBER

HS_CA_MT_UU_G_U_N_0827-2

DEFINITION

fibronectin IIICS

REFERENCE

Hershberger, R. P., and Culp, L. A., Cell-type-specific expression of alternatively spliced human fibronectin IIICS mRNAs. Mol Cell Biol, (1990) 10(2), 662-71.

EXPRESSION

expressed in a variety of human normal and tumor cell types as well as in human liver

REGULATORY FEATURES

ND

TEMPORAL REGULATION

ND

TECHNIQUE

novel double primer extension assay (DPE)

ACCESSION

X04530

SOURCE

genomic DNA

5' SCORE

9.55

3' SCORE

10.97

ACCEPTOR SITE

ttccttccacag

EXON SEQUENCE

3 acgagcttcc ccaactggta acccttccac accccaatct tcatggacca gagatcttgg atgttccttc cacagttcaa aagacccctt tcgtcaccca ccctgggtat gacactggaa atggtattca gcttcctggc acttctggtc agcaacccag tgttgggcaa caaatgatct ttgaggaaca tggttttagg cggaccacac cgcccacaac ggccaccccc ataaggcata ggccaagacc atacccgccg aatgtag

DONOR SITE

gtgagg

5' EXON

na

3' EXON

na

DISEASE

no

COMMENTS

see: Vibe-pedersen, K., Magnusson, S., Baralle, F.E., Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing, FEBS Lett. (1986) 207:287-291. ;; The authors describe a short region of the FN gene with a complex splicing variation including exon subdivision of the 5'-end of an exon and a short intron which is not always removed. This is the third place where differential splicing has been observed so far in the human FN gene transcript and it represents a new type of differential splicing. ;; Oyama, F., Murata, Y., Suganuma, N., Kimura, T., Titani, K., and Sekiguchi, K., Patterns of alternative splicing of fibronectin pre-mRNA in human adult and fetal tissues. Biochemistry, (1989) 28(3), 1428-34. ;; Alternative splicing of fibronectin pre-mRNA at two distinct regions, termed ED-A and IIICS, was investigated with human adult and fetal tissues by the nuclease S1 protection assay. Results strongly suggest that the alternative splicing of fibronectin pre-mRNA in vivo is regulated in a tissue type specific manner at both the ED-A and IIICS regions and that it is developmentally regulated in liver but not in lung.

REVISION

This entry is created on 970316